formulated on the day of administration. PGC-GLP-1 were provided by PharmaIN Corporation in septum vials, resuspended as needed with sterile 0.9% Salvianic acid A web sodium chloride, and stored for up to three weeks at 2-8C. PGC stands for protected graft copolymer made up of 15-30 kDa polylysine in which 55% of epsilon amino groups linked to 5kDa Methoxy polyethylene glycol and the remaining amino groups linked with stearic acid. The pharmacokinetic behavior PGC-GLP-1 formulation was tested in mice prior to shipment as part of several quality control processes and the results were consistent with the paper cited above. Tregitope peptides and liposomes were provided as concentrated stock solutions at -20C or 2-8C, respectively, which were diluted and mixed in the appropriate combinations on each day of administration. Hamster anti-mouse CD3 IgG F2 or isotype control was screened for prevalent rodent infectious agents, and aliquoted into single use vials. Aliquots were thawed as needed and diluted in sterile 1x PBS on the day of administration. preserved in neutral-buffered formalin for light microscopy studies. Animals were exposed to a 12-hr light/dark light cycle. BRM BBDP and MAD rat colonies were kept at sufficient sizes to ensure adequate numbers of rats to enter onto study in a single enrollment period as described below. NOD mice were purchased from The Jackson Laboratory in pre-study cohorts ranging in size from 100-200 mice; these 9057848 cohorts were then used to enroll onto studies as described below. Ethics Statement The veterinary care of the animals were in accordance with Test Facility’s standard operating 
procedures, and regulations outlined in the applicable sections of the Final Rules of the Animal Welfare Act regulations, the Public Health Service Policy on Humane Care and Use of Laboratory Animals, the Guide for the Care and Use of Laboratory Animals, and the BRM, Inc. Policy on Humane Care. The study protocols and any amendments or procedures involving the care or use of animals in studies were reviewed and approved by the Test Facility’s Institutional Animal Care and Use Committee before the initiation of such procedures. The following approved BRM IACUC Docket numbers cover these studies: 06-16, 09-07, 09-23, and 09-30. Experimental Procedures 1) For prevention studies using BBDP rats, male rats were selected at 25 or 30 days of age for the DT22669 or CsA study, respectively, and enrolled into groups in a staggered fashion. BBDP rats were administered 0.2 mg/kg CsA or vehicle once daily by IP injection from 30-60 days of age, or 100 mg/kg DT22669 or vehicle once daily by oral gavage for the duration of the study. BBDP rats were monitored for diabetes onset three times weekly until 120 days of age. Rats were euthanized within 2 days of diabetes onset or at completion of study. 2) For prevention studies using 22634634 the inducible MAD rat, male and female rats 21-25 days of age at initiation of diabetes induction. To induce diabetes, each rat was administered 3 g/g poly by IP injection on Days -3, -2, and -1, followed by an IP injection of 1×107 PFU of Kilham rat virus on Day 1. The induction conditions were extensively optimized and reported previously. ISO-092 or vehicle was administered via overnight-primed ALZET osmotic pumps implanted on Day -1. As a positive control, a short course of dexamethasone was administered by IP injection on Days 6-10. Pumps were replaced on Day 22. Glycosuria was monitored three times weekly and positive tests were fol
Posted inUncategorized