Or vehicle. In either case C60 exposure exacerbated myocardial infarction. Inside each and every PKCβ Modulator Molecular Weight delivery route, infarct sizes within the male groups had been bigger than those in females. In between delivery routes, the females had bigger infarctions in response to IT C60 exposure compared with IV exposure. p 0.05 by two-way ANOVA, N = 4?.exposed to IV C60 when compared with serum collected from male rats exposed to IV automobile and male rats exposed to IT C60 (Fig. 4A). Female rats exposed to IV C60 had considerably reduced serum IL-6 concentrations than IV C60 exposed males. Serum IL-6 concentrations have been unaltered amongst all IT groups and IV exposed females. MCP-1 showed a equivalent profile to IL-6. MCP-1 concentrations had been higher in serum-collected post-I/R from male rats exposed to C60 IV when compared with serum collected from male rats exposed to IV automobile, male rats exposed to IT C60 and female rats exposed to C60 IV (Fig. 4B). Female rats exposed to IV C60 had significantly decrease serum MCP1 concentrations than IV C60 exposed males. Serum MCP-1 concentrations were unaltered in between all IT groups and IV exposed females. VEGF was variable and slightly elevated in females exposed to IT and IV C60 , though not considerably dif-TABLE two Rat Pulmonary Responses to Intratracheal (IT) or Intravenous (IV) Delivery of C60 or Automobile SuspensionsTotal bronchoalveolar lavage fluid (BALF) P2Y2 Receptor Agonist Accession protein and cellularity BAL samples collected 24 h following exposure in males, data expressed as mean ?SEM Protein ( g/ml) IT Car 560 ?149 C60 316 ?55 Na�ve i 456 ?31 n four? p 0.05 versus IT C60. IV 638 ?100 750 ?138 Cell number (?105 ) IT 5.7 ?1.four 3.9 ?0.8 five.6 ?0.5 four? IV three.five ?0.9 six.9 ?1.7 Macrophages (?105 ) IT five.three ?1.three 4.0 ?0.7 five.six ?0.five three? IV five.7 ?0.7 two.5 ?1.two Neutrophils (x 103 ) IT 14.2 ?10 13.3 ?12 7.7 ?four.2 3? IV two.1 ?0.3 1.7 ?1.4 Eosinophils (?03 ) IT 1.9 ?1.1 0.five ?0.five 1 three? IV 2.four ?2.four 1 Epithelial cells (?103 ) IT six.six ?two.four two.8 ?0.three eight.7 ?four.0 3? IV 1.7 ?1.7 8.two ?two.6 CARDIOVASCULAR INJURY IN RESPONSE TO CTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in females, information expressed as mean ?SEM Protein ( g/ml) Automobile C60 Na�ve i n IT 252 ?28 206 ?24 IV 195 ?eight.2 226 ?41 N/A 5? Cell quantity (?105 ) IT IV 7.eight ?1.9 four.1 ?0.six 7.1 ?1.2 three.5 ?0.5 six.six ?1.0 4? Macrophages (?105 ) IT IV 1.6 ?0.9 3.3 ?0.4 0.9 ?0.3 3.1 ?1.1 six.6 ?1.1, four? Neutrophils (?103 ) IT 35.0 ?25.three 27.five ?11.1 IV 1 1 Eosinophils (?103 ) IT IV 0.25 ?0.25 1.7 ?1.7 12.5 ?7.5 3.6 ?0.six 1 four? Epithelial cells (?103 ) IT IV 132 ?90 346 ?132 75.0 ?33 362 ?59 179 ?43 four?1 4?N/A, not applicable. p 0.05 versus IT C60 . p 0.05 versus IT vehicle.THOMPSON ET AL.TABLE 3 Rat Coronary Artery Pharmacological Responses for Serotonin (5-HT), ACh, SNP, and ET-Male rat EC50 s [nM] (imply ?SEM) IT delivery C60 5-HT ACh SNP ET-1 559 ?104 265 ?45 116 ?24 38 ?20 Vehicle 871 ?130 239 ?34 76 ?18 48 ?15 IT delivery 5-HT ACh SNP ET-1 C60 1.7 ?0.1 2.6 ?0.four 2.4 ?0.6 1.three ?0.2 Automobile three.2 ?0.7 1.7 ?0.1 two.2 ?0.three 1.0 ?0.05 IT delivery 5-HT ACh SNP ET-1 C60 813 ?128 1419 ?843 19 ?3 29 ?20 Car 1268 ?497 139 ?28 21 ?4 6.5 ?2.4 IT delivery 5-HT ACh SNP ET-1 C60 1.four ?0.4 1.0 ?0.four 1.six ?0.three 1.four ?0.3 Car 1.0 ?0.two 1.six ?0.three 2.1 ?0.4 1.1 ?0.three p-value, n 0.39, five 0.29, 5 0.35, four 0.41, five C60 1.three ?0.four 1.0 ?0.2 two.0 ?0.three 1.1 ?0.two p-value, n C60 0.ten, 4 2.0 ?0.2 0.ten, 4 3.8 ?0.eight 0.77, four 2.1 ?0.three 0.09, 6 1.1 ?0.1 Female rat EC50 s [nM] (mean ?SEM) p-value, n 0.06, four 0.33, 4 0.13, 4 0.49, six C60 IV deliv.
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