vels of glutathione than healthy individuals. Institutional ethical clearance was obtained for the study and informed consent was obtained from each subject recruited in the study. The subjects were grouped into two groups; Group I-15 healthy subjects in control group without any habit and oral lesions. Group II-30 patients who were clinically and histologically diagnosed with squamous cell carcinoma of the oral cavity and 
who had not received any previous treatment. Readings of group II were again taken at postoperatively at 1st month and 6th month respectively from all these 30 patients, biopsies were taken from the deepest and most prominent part of the lesion and were subjected to histopathological evaluation of haematoxylin and eosin. These stained 123 J Maxillofac Oral Surg 8:270274 271 Test & Control-Unpaired t-test sections were graded according to Bryne’s invasive front grading system. Method for estimation of glutathione in blood samples: 4.0 ml of the venous blood from each subject was drawn from the medial cubital vein by using 24 gauge needles in a sterile PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19801058 disposable syringe set under aseptic measures. The blood was then transferred to 0.5 M-EDTA – containing bottles to prevent coagulation. 4ml of blood is added to a test tube containing 2.5 ml of histopaque-1077 and then centrifuged for 20 minutes at 3000 rpm. Erythrocytes were separated out carefully and preserved in cryovials at -80C in refrigerator. t df P-value Mean difference Std. Error difference Procedure 1. 0.1 ml of erythrocytes were taken into test tube and added to 1.9 ml of distilled water. 2. Then 3 ml of precipitating solution was added to it, centrifuged for 8 minutes and filtered thereafter. 3. 2 ml of filtrate was taken and mixed with 8 ml of phosphate solution. 4. Then, 1 ml of DTNB solution is added to the whole mixture. Yellowish color appeared. 5. Optical density of the solution was then measured within 10 minutes at 412 nm using a spectrophotometer. This method is based upon the development of a relatively stable yellow color when 5, 5′ – dithiobis is added to sulfhydryl compounds including glutathione. In this invasive front tumor five different histological features are graded and assigned points from 1 to 4, the score for each variable is summed to provide a total malignancy score for each tumor. The scores from 49, 10 15, 1620 would represent for well, moderately and poorly differentiated oral squamous cell carcinomas, respectively Results In the present study the mean of glutathione concentration values in erythrocytes for oral squamous cell carcinoma patients was 78.40050 and for control group was 90.87280. There is a difference of 12.472 i.e., 14.44% between control and patient samples as shown in Preoperative Postoperative 1 month Postoperative 6 months 78.4013 76.9763 80.0030 male and female group is 78.960 and 76.561 respectively. The difference between two groups is of 2.399 i.e., 2.54%. But on statistical analysis the difference was found to be insignificant. So there is no gender predilection. Patients were AZD 0530 scored in the range of 49, 1015, >15 histopathologically according to the Bryne’s invasive front grading system. Our 123 272 J Maxillofac Oral Surg 8:270274 study has 9 patient samples with scores in the range of 49, 19 patient samples with scores in the range 1015 and 2 patient sample with scores >15. So the mean glutathione concentration value for well differentiated, moderately differentiated and poorly differentiated is 80.554, 77.
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