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P-glycoprotein monoclonal antibody (C219)
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| Alternative Name ABCB1, CD243, P-glycoprotein 1 | Application ICC, IHC (FS), IHC (PS), IP | Application Notes Flow Cytometry: cell permeabilization required.Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot.{{320-67-2} site|{320-67-2} Protocol|{320-67-2} Data Sheet|{320-67-2} manufacturer} Cited applications include ELISA, IF, FC, and WB | Clone C219 | Crossreactivity Cross-reacts with a ~200kDa protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2). | Formulation Phosphate-buffered solution with BSA and 0.{{81525-13-5} site|{81525-13-5} Technical Information|{81525-13-5} Formula|{81525-13-5} custom synthesis} 09% NaN3 | Host Mouse | Immunogen SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.PMID:31194403 | Isotype IgG1κ | Positive Control Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.Tissue: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretory epithelium) are recommended. | Recommendation Dilutions/Conditions Immunohistochemistry (paraffin sections):- For optimal staining, the sections should be pretreated with an antigen unmasking solution.- The antibody may be diluted to ≥1:25 for biotin based detection systems.- For optimal staining, the primary antibody should be incubated 60 minutes at room temperature.Western Blot (1:50-1:100)Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. | Species Reactivity Dog, Human, Mouse, Primate, Rat | Specificity Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein. | UniProt ID P08183 | Unit of Measure (UM) ml