Product Name :
Anti-Rat IgG(H+L), Goat antibody(iFluor647)
Applications:
ELISA,WB,ICC/IF,Flow Cyt
Reactivity :
Rat IgG(H+L)
Conjugate:
iFluor647
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Rat IgG(H+L), Goat antibody(iFluor647) is designed for detecting rat IgG specifically. Anti-Rat IgG(H+L), Goat antibody(iFluor647) is based on recombinant, goat IgG Fc fused single domain antibody to rat IgG coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(H+L), Goat antibody(iFluor647) reacts with rat IgG selectively. | Immunogen: Rat IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation) | Conjugate: iFluor647(Ex: 652nm, Em: 668nm), 3 moles iFluor647 per mole IgG | Specificity: Rat IgG(H+L) | Cross-Reactivity: Does not bind to mouse IgG, rabbit IgG, goat IgG, human IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.{{216974-75-3} site|{216974-75-3} Technical Information|{216974-75-3} Data Sheet|{216974-75-3} manufacturer} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light. | Background:There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from rat. Each isotype has a different heavy chain. Rat IgG consists of four subclasses-IgG1, IgG2a, IgG2b, IgG2c. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site.{{52232-67-4} site|{52232-67-4} Biological Activity|{52232-67-4} In Vivo|{52232-67-4} custom synthesis} The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD.PMID:25905396 The common IgG is monomeric with a molecular weight of approximately 150 kD.Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-Rat IgG(H+L), Goat antibody(iFluor647) is designed for detecting rat IgG specifically. Anti-Rat IgG(H+L), Goat antibody(iFluor647) is based on recombinant, goat IgG Fc fused single domain antibody to rat IgG coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(H+L), Goat antibody(iFluor647) reacts with rat IgG selectively.
Immunogen:
Rat IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Function:
Flow Cyt: 1:200-1:2000ICC/IF： 1:200-1:2000ELISA: 1:5000 -1:20000WB: 1:5000 -1:20000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.