Inc. Published in the U.S.A.Arabidopsis Ferritin 1 (AtFer1) Gene Regulation by the Phosphate Starvation Response 1 (AtPHR1) Transcription Factor Reveals a Direct Molecular Link involving Iron and Phosphate Homeostasis*SReceived for publication, May well 1, 2013, and in revised type, June 19, 2013 Published, JBC Papers in Press, June 20, 2013, DOI ten.1074/jbc.M113.Marc Bournier, Nicolas Tissot, St hane Mari, Jossia Boucherez, Eric Lacombe Jean-Fran is Briat, and Fr ic Gaymard1 From the Laboratoire de Biochimie et Physiologie Moleculaire des Plantes, UMR 5004, Agro-M/CNRS/Institut National de la Recherche Agronomique/Universite Montpelier II, 34060 Montpellier Cedex 1, France plus the �Department of Plant Resistance to Pests, IRD, 911 av Agropolis, BP 64501, 34394 Montpellier Cedex five, FranceBackground: Physiological evidences have linked phosphate and iron nutrition in plants. Final results: Both PHR1 and PHL1 interact with AtFer1 promoter area and regulate its expression in an iron-independent manner. Conclusion: A molecular link exists amongst the handle of iron and of phosphate homeostasis. Significance: PHR1 and PHL1 play a essential role within the regulation of each phosphate and iron homeostasis. A yeast one-hybrid screening permitted the collection of PHR1 as a aspect that interacted together with the AtFer1 ferritin gene promoter.Bemnifosbuvir In mobility shift assays, PHR1 and its close homologue PHL1 (PHR1-like 1) interact with Element 2 from the AtFer1 promoter, containing a P1BS (PHR1 binding website).Loratadine Within a loss of function mutant for genes encoding PHR1 and PHL1 (phr1 phl1 mutant), the response of AtFer1 to phosphate starvation was entirely lost, displaying that the two transcription things regulate AtFer1 expression upon phosphate starvation. This regulation doesn’t involve the IDRS (iron-dependent regulatory sequence) present in the AtFer1 promoter and involved in the iron-dependent regulation. The phosphate starvation response of AtFer1 just isn’t linked towards the iron status of plants and is specifically initiated by phosphate deficiency. Histochemical localization of iron, visualized by Perls DAB staining, was strongly altered within a phr1 phl1 mutant, revealing that each PHR1 and PHL1 are main variables involved in the regulation of iron homeostasis.As a result of its redox properties, iron can be a big cofactor for many proteins involved in several biological processes such as photosynthesis or respiration. However, its capability to simply gain or drop electrons tends to make it extremely reactive with oxygen and potentially toxic. This duality of iron imposes a tight regulation of its homeostasis to allocate a adequate quantity for metabolism and to stop an excess deleterious for cell integrity.PMID:24187611 Plants have evolved quite a few methods to maintain iron homeostasis, like checkpoints of its absorption, allocation, and chelation. Within this context, the recent identification of a number of transcription issue cascades activating iron uptake in response to iron deficiency represented a significant breakthrough* This function was supported by the Centre National de la Recherche Scientifique (CNRS) (to M. B.). This article consists of supplemental Table S1. 1 To whom correspondence needs to be addressed: 2 place Viala, 34060 Montpellier Cedex 1, France. Tel.: 33-499-612-932; Fax: 33-467-525-737; E-mail: [email protected](1). In contrast, considerably less is identified concerning the regulation of expression of genes involved within the buffering and storage of iron when in excess. In plants, ferritins are plas.
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