Francesca. Pediatric team: Buonsenso Danilo, Valentini Piero, Sinatti Dario, De Rose Gabriella. Pneumology team: Richeldi Luca, Lombardi Francesco, Calabrese Angelo, Varone Francesco, Leone Paolo Maria, Siciliano Matteo, Corbo Giuseppe Maria, Montemurro Giuliano, Calvello Mariarosaria, Intini Enrica, Simonetti Jacopo, Pasciuto Giuliana, Adiletta Veronica, Sofia Carmelo, Licata Maria Angela. Psychiatry team: Sani Gabriele, Delfina Janiri, Simonetti Alessio, Modica Marco, Montanari Silvia, Catinari Antonello, Terenzi Beatrice. Radiology group: Natale Luigi, Larici Anna Rita, Marano Riccardo, Pirronti Tommaso, Infante Amato. Rheumatology group: Paglionico Annamaria, Petricca Luca, Tolusso Barbara, Alivernini Stefano, Di Mario Clara. Vascular illness team: Santoliquido Angelo, Santoro Luca, Nesci Antonio, Di Giorgio Angela, D’Alessandro Alessia. Conflicts of Interest: The authors declare no conflict of interest. The L-arginine plus vitamin C supplement and placebo have been donated by Farmaceutici Damor, Naples, Italy. The supplier had no function in the design of the study; inside the collection, analyses, or interpretation in the data; inside the writing of your manuscript; or in the decision to publish the outcomes.
Chinese Herbal Medicines 15 (2023) 86Contents lists readily available at ScienceDirectChinese Herbal Medicinesjournal homepage: elsevier/locate/chmedOriginal ArticleProtective effect of hydroxysafflor yellow A on MSCs against senescence induced by D-galactoseXiaoqing Song , Jinying Wang, Yu Zhang, Xinqian Du, Qibing QianBasic Medical College of Hebei North University, Zhangjiakou 075000, Chinaa r t i c l ei n f oa b s t r a c tObjective: To examine the protective effects of hydroxysafflor yellow A (HSYA) against the senescence of mesenchymal stem cells (MSCs) induced by D-galactose (D-gal) in vitro, and investigate the possible mechanism involved. Approaches: Grouping experiment, Normal control (NC) group: standard culture with full medium; Senescence group: MSCs had been cultured for 48 h with complete medium containing ten g/L D-gal; HSYA group: on the basis of senescence induction, HSYA together with the suitable concentration was applied to safeguard MSCs. The important experimental indices linked with oxidative tension, inflammatory response, cell senescence, proliferation and apoptosis had been measured by way of chemical colorimetry, b-galactosidase staining, EdU incorporation and flow cytometry, respectively.Kisspeptin-10, human manufacturer The relative quantity (RQ) of proteins connected closely to cell proliferation, apoptosis, and NF-jB signaling were measured by Western blotting.AICAR MedChemExpress Benefits: As compared with Senescence group, treatment with HSYA (120 mg/L) effectively ameliorated the adverse scenario of MSCs.PMID:24631563 Oxidation anxiety and inflammation in conjunction with D-Gal induction was drastically alleviated in MSCs; The b-Gal-positive staining indicated that MSC senescence was drastically mitigated; The proliferative capability of MSCs was considerably increased by up-regulating PCNA and inhibiting p16 expression; The anti-apoptotic effect on MSCs was exerted by down-regulating the RQ of cleaved Caspase-3 and Bax; The activity of NF-jB signaling in MSCs was notably suppressed by way of inhibiting phosphorylation of IKKb and p65. Conclusion: HSYA (120 mg/L) drastically delayed the D-Gal-induced senescence approach in MSCs by way of attenuating inflammatory reaction and oxidative anxiety, and suppressing the activity of NF-jB signaling. 2022 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V. Th.
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