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(2021) 22:899 Sun et al. BMC Genomics doi.org/10.1186/s12864-021-08213-wRESEARCHOpen AccessTranscriptome comparative analysis of ovarian follicles reveals the important genes and signaling pathways implicated in hen egg productionXue Sun1,two, Xiaoxia Chen1,2, Jinghua Zhao1,two, Chang Ma1,2, PKCα Accession Chunchi Yan1,2, Simushi Liswaniso1,2, Rifu Xu1,two and Ning Qin1,2Abstract Background: Ovarian follicle improvement plays a crucial part in determination of poultry egg production. The follicles at the numerous developmental stages possess their own distinct molecular genetic traits and have unique biological roles in chicken ovary improvement and function. Inside the every single stage, numerous genes of follicle-specific expression and biological pathways are involved within the vary-sized follicular improvement and physiological events. Identification from the pivotal genes and signaling pathways that manage the follicular development is beneficial for understanding their precise regulatory functions and molecular mechanisms underlying egg-laying traits of laying hens. Benefits: The comparative mRNA transcriptomic evaluation of ovarian follicles at three important developmental stages such as slow growing white follicles (GWF), smaller yellow follicles (SYF) of recruitment in to the hierarchy, and differentiated large yellow follicles (LYF), was achieved inside the layers with lower and larger egg production. Completely, 137, 447, and 229 of up-regulated differentially expressed genes (DEGs), and 99, 97, and 157 of down-regulated DEGs inside the GWF, SYF and LYF follicles, such as VIPR1, VIPR2, ADRB2, and HSD17B1 had been identified, respectively. Additionally, NDUFAB1 and GABRA1 genes, two most promising candidates potentially associated with egg-laying overall performance have been screened out from the 13 co-expressed DEGs inside the GWF, SYF and LYF samples. We further investigated the biological effects of NDUFAB1 and GABRA1 on ovarian follicular improvement and found that NDUFAB1 promotes follicle improvement by stimulating granulosa cell (GC) proliferation and decreasing cell apoptosis, increases the expression of CCND1 and BCL-2 but attenuates the expression of caspase-3, and facilitates steroidogenesis by enhancing the expression of STAR and CYP11A1. In contrast, GABRA1 inhibits GC proliferation and stimulates cell apoptosis, decreases the expression of CCND1, BCL-2, STAR, and CYP11A1 but elevates the expression of caspase-3. Additionally, the 3 crucial signaling pathways for instance PPAR signaling pathway, cAMP signaling pathway and neuroactive ligand-receptor interaction were drastically enriched, which may play important roles in ovarian follicle growth, differentiation, follicle selection, and maturation.Correspondence: [email protected]; [email protected] Xue Sun and Xiaoxia Chen contributed equally to this perform. two Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China Full list of author facts is readily available at the end of your articleThe Author(s) 2021. Open Access This article is licensed below a Inventive Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, provided that you give acceptable TLR1 Formulation credit for the original author(s) plus the supply, give a hyperlink for the Creative Commons licence, and indicate if alterations have been made. The photos or
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