Earing loss.Author Contributions: Conceptualization, S.-Y.K.; methodology, S.-Y.K.; formal evaluation, S.-Y.K., S.-M.L., and K.-W.K. writing–original draft preparation, K.-J.C. and S.-Y.K.; writing–review and editing, K.-J.C., S.-Y.K., C.-H.L., K.-W.K., and S.-M.L.; funding acquisition, S.-Y.K. and C.-H.L. All authors have read and agreed for the published version of the manuscript. Funding: This study was supported by funding from the National Investigation Foundation (NRF) of Korea (NRF-2018R1D1A1B07048092 (Approval date: 1 May perhaps 2018) and 2020R1A2C4002594 (Approval date: 1 March 2020)). The APC was funded by 2020R1A2C4002594. Institutional Evaluation Board Statement: The Institutional Animal Care and Use Committee of CHA University (IACUC200025) approved the performed animal experiments. The circumstances of animal rearing, drug administration, and sacrifice complied using the regulations in the Institutional Animal Care and Use Committee of CHA University. Informed Consent Statement: Not applicable.Int. J. Mol. Sci. 2021, 22,11 ofData Availability Statement: The information presented within this study are readily available upon request from the corresponding author. Conflicts of Interest: The authors declare no conflict of interest. The funders had no role within the style with the study; in the collection, analyses, or interpretation of information; within the writing with the manuscript, or in the choice to publish the outcomes.
Metabolic Engineering Communications 13 (2021) eContents lists out there at ScienceDirectMetabolic Engineering Communicationsjournal homepage: www.elsevier.com/locate/mecMetabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction with the sesquiterpene Adenosine A1 receptor (A1R) Agonist Compound valenceneMaximilian Dietsch a, 1, Anna Behle a, 1, Philipp Westhoff b, Ilka M. Axmann a, a bInstitute for Synthetic Microbiology, Department of Biology, Heinrich Heine University D seldorf, D seldorf, Germany Plant Metabolism and Metabolomics Laboratory, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University D seldorf, D-40001, D seldorf, GermanyA R T I C L E I N F OKeywords: Metabolic engineering Cyanobacteria Synechocystis Valencene SesquiterpeneA B S T R A C TCyanobacteria are very adaptable, fast-growing, solar-powered cell factories that, like plants, are able to convert carbon dioxide into sugar and oxygen and thereby produce a sizable number of vital compounds. Due to their exclusive phototrophy-associated physiological properties, i.e. naturally occurring isoprenoid metabolic pathway, they represent a highly promising platform for terpenoid biosynthesis. Right here, we implemented a carefully devised engineering technique to boost the biosynthesis of commercially attractive plant sequiterpenes, in distinct valencene. Sesquiterpenes are a diverse group of bioactive metabolites, mainly developed in greater plants, but with usually low Trypanosoma Compound concentrations and costly downstream extraction. Within this function we successfully demonstrate a multi-component engineering method towards the photosynthetic production of valencene inside the cyanobacterium Synechocystis sp. PCC 6803. Initial, we improved the flux towards valencene by markerless genomic deletions of shc and sqs. Secondly, we downregulated the formation of carotenoids, which are necessary for viability in the cell, working with CRISPRi on crtE. Finally, we intended to improve the spatial proximity of your two enzymes, ispA and CnVS, involved in valencene formation by making an operon construct, as well as a fusion protein. Combining t.
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