Rstained with hematoxylin or incubated with Alexa-fluor conjugated secondary antibodies (Invitrogen) for 2h, washed with
Rstained with hematoxylin or incubated with Alexa-fluor conjugated secondary antibodies (Invitrogen) for 2h, washed with TBS-T, counterstained with DAPI and coverslipped.Human primary aortic VSMC (Lonza) had been employed between passages…