E Phase C 85.0 80.0 76.0 71.0 70.0 69.0 68.5 68.0 67.five 66.2.5. Preparation of Test Answer 2.5.1. Preparation of Standard Reference Remedy An amount of three.two mg of benzoic acid was poured into a 10 mL brown volumetric flask. Then chromatographically pure methanol solution was added towards the mark to obtained a reference stock answer with a Concentration of 0.32 mg L-1 . 2.five.2. Preparation of Test Solution The dried roots of C. paliurus have been pulverized and passed by way of a Pharmacopoeia sieve having a pore size of 0.15 mm for experiments. The response surface style of your extraction of benzoic acid from C. paliurus gave the optimal condition, which can be with 60 methanol answer as extraction solvent plus the ratio of liquid to material was 25:1 (mL/g) at 60 C for 60 min [36]. Based on the above extraction condition, 3 g of roots powder was transferred to a 100 mL conical flask, which contained 75 mL of methanol solution. Then the mixture was sonicated for 60 min. Right after cooled to space temperature, it was suction filtered along with the Gardiquimod Autophagy filtrate was produced as much as a one Iproniazid Data Sheet hundred mL volumetric flask and stored within a dark spot. 2.5.three. Determination of Sample Determination Time Taking the benzoic acid common option as the test option and performing HPLC evaluation beneath the chromatographic circumstances of “2.4”, a single-needle injection on the benzoic acid typical solution was determined for its peak time (Supplementary Material).Chemistry 2021,two.five.4. Investigation of Linear Partnership The benzoic acid normal remedy was accurately extracted by 0.5 , 1.0 , 1.5 , 2.0 , five.0 , and 10.0 . The peak area was measured by injection below the above chromatographic circumstances. Taking the mass in the regular handle as the independent variable plus the peak region because the dependent variable, the regression equation was obtained as follows: y = 5394.9x – 24.69, R2 = 0.9996. The linear range is 0.016 0.320 mg/mL. two.six. Adsorption Experiment of ECM on Benzoic Acid in C. paliurus Extract 2.six.1. Static Adsorption Experiments Static adsorption tests on EC and ECM absorbents have been performed as follows: 25 mL extracting remedy of C. paliurus was added to mix with pre-weighed two.0 g dry absorbents in the flasks. The flasks were sealed tightly and shaken on an incubation shaker (120 rpm) at 25 C for 24 h to attain adsorption equilibrium. The option following adsorption was gained by filtration and subjected to further HPLC analysis. 2.6.2. Adsorption Kinetic The experiment was timed in the moment that the ECM adsorbents (two.0 g) in flask was added towards the C. paliurus answer (25 mL) of benzoic acid. The concentration of benzoic acid common resolution was 0.010 mg/mL. Subsequently, the flask was continually shaken in an incubator shaker (120 rpm) at 25 C. The benzoic acid concentration in the adsorption method was determined with HPLC at frequent intervals until the equilibrium was reached. 2.7. Desorption Performance Test following Adsorption Equilibrium of ECM two.7.1. The Influence of Methanol Concentration on the Desorption Performance of Benzoic Acid Because of the influence of methanol concentration on benzoic acid desorption, overall performance is greater than ultrasonic time and temperature, and so forth. At the identical time, the loss of ECM increases with rising of methanol concentration. Consequently, the influence of 30 , 40 , 50 , 60 , and 70 methanol on desorption results is mostly discussed. The experimental circumstances have been as follows: Unique concentrations of methanol have been made use of.
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