Day: January 12, 2021

Esuspended in homogenization buffer (1 M HEPES with 1 M sucrose; Life Technologies) containing protease inhibitors cocktail (Sigma). THP-1 cells had been then mechanically lysed by many passages by way…

Med utilizing the exact same patch clamp intracellular answer in which EGTA was substituted by the calcium sensitive dye Fluo-4 (one hundred , Molecular Probes-Invitrogen, France). Following at the least…