Day: June 27, 2018

Minutes. The supernatant was discarded and the pellet resuspended in trans-ACPD buffer A (50 mM Tris, 2 mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for…

Minutes. The supernatant was discarded as well as the pellet resuspended in buffer A (50 mM Tris, two mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37…