Hsp70-white reporter lines with variegating eye phenotype are excluded from regions related with Pc, and DNase I hypersensitive sites (DHS) are associated with genes within the Computer domains. For hsp70-w reporters, red bars denote insertions with red eye phenotype (full expression), while black bars denotes insertions with variegating eyes. (PDF)Figure S3 Distribution of chromosomal proteins and histone marks unique to chromosome 4 in S2 cells. Metagene evaluation for the enrichment (averaged smoothed M-values, Y-axis) for selected marks is plotted against position relative for the TSS for any 3 kb scaled metagene (bp, X-axis). The enrichment is examined separately for active (left) and repressed (proper) genes in 3 genomic domains, chromosome four (leading panel), pericentric heterochromatin (middle panel), and euchromatin (bottom panel), together with the number of genes for every single category illustrated at the ideal corner. (PDF) Figure S4 Chromosome four genes exhibit special chromatin marks compared to genes in heterochromatin and euchromatin in BG3 cells. Similar analysis as shown in Figure 3, now together with the exact same number of genes (N) as present on chromosome 4 randomly chosen from heterochromatin (Hetero) and euchromatin (Eu) as controls. (PDF) Figure S5 Chromosome four genes exhibit exceptional chromatin marks in comparison to genes in heterochromatin and euchromatin in S2 PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20030704 cells. Identical evaluation as shown in Figure S3, now using the very same number of genes (N) as present on chromosome four randomly chosen from heterochromatin (Hetero) and euchromatin (Eu) as controls. (PDF) Figure S6 Heatmap showing the enrichment of select chromosomal proteins and histone marks at genes on chromosome 4, when compared with heterochromatin and euchromatin. A. BG3 cells. B. S2 cells. The region around the TSS and TTS (+/2500 bp) is just not scaled, although the gene physique is scaled. For that reason, only genes longer than 1 kb are regarded right here. Enrichment is shown in red, depletion in blue. Eu – euchromatin. Hetero – heterochromatin. (PDF) Figure S7 Histogram of the expected incidence of RNA pol IIValidation of ChIP-chip HP1a and POF enrichment peaks. A. Fraction of HP1a peaks decreased in HP1a mutants (third instar larvae). 96 of peaks are substantially lowered. X-axis: Mvalue of HP1a peaks in WT; Y-axis: fraction of peaks reduced inside the mutants. B. Fraction of POF peaks decreased in POF mutants (third instar larvae). 79 of peaks are substantially decreased. Xaxis: M-value of POF peaks in WT; Y-axis: fraction of peaks decreased inside the mutants. (PDF)Figure SEffect of HP1a depletion on RNA pol II pausing index. A. Ratio of the PI in mutants lacking HP1a when compared with wildtype. The PI is defined as the ratio in between the maximum enrichment value around TSS (+/2300 bp) as well as the medium enrichment RG7800 site values over the gene physique (600 bp downstream of TSS to the finish from the gene) [31]. 53 of 74 genes show a rise in PI, indicated by a ratio larger than 1. B. Histogram of RNA pol II level fold modifications in HP1a mutants (log 2; average per gene) for genes on chromosomes two, 3, and X (bottom panel) and genes on chromosome four (prime panel), illustrating a reduce of RNA pol II levels for chromosome 4 genes. C. Partnership among RNA pol II level modifications (Y-axis, in log two) and expression level adjustments (Xaxis, in log 10) of chromosome 4 genes in HP1a mutants compared to wildtype. Information points with x,0 and y,0 correspond to genes where each RNA pol II and expression levels decrease upon HP1a depletion (67 of 84 genes on chromosome 4; r = 0.four, Pearson co.
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