Ed of four rungs of b-strands, based on their interpretation of

Ed of four rungs of b-strands, based on their interpretation of X-ray diffraction patterns [28]. In this model, each rung would comprise ,36?7 residues. Positions N152-M153 lie near the middle of the G85-S232 sequence, so it is tempting to speculate that they might be located at an exposed position at the border between rungs. This might explain why the N152-S232 and/or M153-S232 fragment emerges as the most conspicuous PK-resistant fragment after prolonged treatment with PK or partial unfolding with guanidine (Figures 4 and 5). Positions A116-G118 might be the border between the two most aminoterminal 15900046 rungs (approximately G85-A115 and A119-E151). On the other hand, our inhibitor results are partially inconsistent with the locationFigure 4. Kinetics of PK digestion of unpurified GPI2 PrPSc. Samples were digested with PK (25 mg/ml) and the reaction stopped after 0, 30, 60, 120, 180, 240, 300 and 360 minutes. Samples were treated with PNGase F and subjected to Tricine-SDS-PAGE the blot was probed with R1 antibody. doi:10.1371/journal.pone.0050111.gStructural Organization of Mammalian PrionsPreparation of Brain Homogenates and Isolation of GPIanchorless PrPScMouse BH, 10 w/v, were prepared in PBS, 5 sarkosyl, using a dounce homogenizer (Wheaton Industries Inc, NJ, USA), followed by one pulse of sonication to clarify the homogenate, with an ultrasonic homogenizer probe (Cole Parmer Instrument CO., Chicago IL, USA). GPI2 PrPSc was isolated using the method of Baron et al. [8]. During the purification, total PrPSc was treated with 10 mg/ml of proteinase K. The final GPI2 PrPSc pellet was resuspended in 100 ml of deionised water or in 20 ml of a 6 M guanidine solution (final concentration 1.75 mg/ml). The stock suspension was stored at 4uC. Its purity was assessed by Coomassie stained SDS-PAGE gel and estimated to 23977191 be ,95 pure. The yield of GPI- PrPSc was ,35 mg per brain (BCA protein assay).Figure 5. Western blot of PK-digested series of GPI2 PrPSc samples following partial unfolding by guanidine HCl. After guanidine partial unfolding with 0 M, 0.5 M, 1 M, 2 M, 3 M and 4 M and PK treatment (25 mg/ml), the samples were treated with PNGase F and resolved on Tricine-SDS-PAGE. The WB was probed with the R1 antibody. doi:10.1371/journal.pone.0050111.gRecombinant PrPRecombinant Mouse PrP(23-231) was expressed in E. coli, and purified and refolded in-column on an NTA affinity column (GE Healthcare, Uppsala, Sweden), as previously described [29]. Refolded protein was dialyzed against 10 mM sodium phosphate buffer pH 5.8 and then against d.i. water.assigned by Govaerts et al., using threading algorithms, to residues K100-P104 and E145-R163, placed in loops and not rungs [10]. Our data show that the stretches formed by residues K100-P104, N142E151, and Y154-Y161, are PK-resistant, i.e., likely part of a b-strand rung (Figure 2 and Table 1). In summary, our data support a PrPSc structure consisting of a series of highly PK-resistant b-sheet strands interspersed with PKsensitive short flexible loops and turns. Furthermore, the region comprising ,V179 to the Epigenetics C-terminus of PrPSc is probably composed primarily of b-sheet, as it is highly resistant to PK. Our data are consistent with our previous results (263K and Dy strains) and those of other researchers using SHaPrPSc. Furthermore, they are consistent with those observed for human CJD PrPSc, which suggests that the myriad human, hamster and mouse prions share a common basic structure.Limited ProteolysisAliquots o.Ed of four rungs of b-strands, based on their interpretation of X-ray diffraction patterns [28]. In this model, each rung would comprise ,36?7 residues. Positions N152-M153 lie near the middle of the G85-S232 sequence, so it is tempting to speculate that they might be located at an exposed position at the border between rungs. This might explain why the N152-S232 and/or M153-S232 fragment emerges as the most conspicuous PK-resistant fragment after prolonged treatment with PK or partial unfolding with guanidine (Figures 4 and 5). Positions A116-G118 might be the border between the two most aminoterminal 15900046 rungs (approximately G85-A115 and A119-E151). On the other hand, our results are partially inconsistent with the locationFigure 4. Kinetics of PK digestion of unpurified GPI2 PrPSc. Samples were digested with PK (25 mg/ml) and the reaction stopped after 0, 30, 60, 120, 180, 240, 300 and 360 minutes. Samples were treated with PNGase F and subjected to Tricine-SDS-PAGE the blot was probed with R1 antibody. doi:10.1371/journal.pone.0050111.gStructural Organization of Mammalian PrionsPreparation of Brain Homogenates and Isolation of GPIanchorless PrPScMouse BH, 10 w/v, were prepared in PBS, 5 sarkosyl, using a dounce homogenizer (Wheaton Industries Inc, NJ, USA), followed by one pulse of sonication to clarify the homogenate, with an ultrasonic homogenizer probe (Cole Parmer Instrument CO., Chicago IL, USA). GPI2 PrPSc was isolated using the method of Baron et al. [8]. During the purification, total PrPSc was treated with 10 mg/ml of proteinase K. The final GPI2 PrPSc pellet was resuspended in 100 ml of deionised water or in 20 ml of a 6 M guanidine solution (final concentration 1.75 mg/ml). The stock suspension was stored at 4uC. Its purity was assessed by Coomassie stained SDS-PAGE gel and estimated to 23977191 be ,95 pure. The yield of GPI- PrPSc was ,35 mg per brain (BCA protein assay).Figure 5. Western blot of PK-digested series of GPI2 PrPSc samples following partial unfolding by guanidine HCl. After guanidine partial unfolding with 0 M, 0.5 M, 1 M, 2 M, 3 M and 4 M and PK treatment (25 mg/ml), the samples were treated with PNGase F and resolved on Tricine-SDS-PAGE. The WB was probed with the R1 antibody. doi:10.1371/journal.pone.0050111.gRecombinant PrPRecombinant Mouse PrP(23-231) was expressed in E. coli, and purified and refolded in-column on an NTA affinity column (GE Healthcare, Uppsala, Sweden), as previously described [29]. Refolded protein was dialyzed against 10 mM sodium phosphate buffer pH 5.8 and then against d.i. water.assigned by Govaerts et al., using threading algorithms, to residues K100-P104 and E145-R163, placed in loops and not rungs [10]. Our data show that the stretches formed by residues K100-P104, N142E151, and Y154-Y161, are PK-resistant, i.e., likely part of a b-strand rung (Figure 2 and Table 1). In summary, our data support a PrPSc structure consisting of a series of highly PK-resistant b-sheet strands interspersed with PKsensitive short flexible loops and turns. Furthermore, the region comprising ,V179 to the C-terminus of PrPSc is probably composed primarily of b-sheet, as it is highly resistant to PK. Our data are consistent with our previous results (263K and Dy strains) and those of other researchers using SHaPrPSc. Furthermore, they are consistent with those observed for human CJD PrPSc, which suggests that the myriad human, hamster and mouse prions share a common basic structure.Limited ProteolysisAliquots o.