O increased in VHL-KO hepatocytes. The protein levels of GLUT1, one of the targets regulated by HIF-1a, were increased in VHL-inactivated hepatocytes; however, the GLUT 2 expression levels were comparable to those of the controls. Unexpectedly, inhibiting the IGF-IR signal with an IGF-IR antagonist sustained the blood glucose levels and remarkably suppressed the hepatic phenotypes. Therefore, the upregulated glucose transporters that were involved in VHL-KO mice were suspected to be GLUT1. In summary, VHL-KO mice exhibited Nobiletin severe hypoglycemia. Along with the severe hypoglycemia and hepatic glycogen accumulation in the liver, hepatic IGF-IR expression was upregulated and the IGF-IR-RACK1 interaction was enhanced. Administering an IGF-IR antagonist resulted in significant improvements in the hypoglycemic state and glycogen accumulation. Our results indicated that IGF-IR was a key regulator of the increased glucose uptake into VHL-deficient hepatocytes. To our knowledge, this is the first study to show that VHL-KO mice have increased glucose uptake into hepatocytes via IGF-IR activation, which contributes to their severe hypoglycemia.Author ContributionsConceived and designed the experiments: AK YK. Performed the experiments: AK YK. Analyzed the data: MF AK YK. Contributed reagents/materials/analysis tools: KI TS TM. Wrote the paper: AK YK.
In the field of infectious diseases, the use of high-content perturbation screens using siRNAs, shRNAs, and chemical agents is 18204824 rapidly expanding. Information regarding cellular factors that assist viruses and other intracellular pathogens during replication 1662274 in the host cell, and on pharmacological agents that affect infection is increasing. To understand disease mechanisms, and to develop novel antiviral strategies, it is important to precisely HIV-RT inhibitor 1 cost define the event in the viral replication cycle that is affected. Knowing the identity of a gene that promotes/inhibits infection, or a drug that blocks infection is not sufficient. Since the number of `hits’ provided by genome-wide and drug screens is generally large, such a method must be high-throughput. In this study, we describe a series of such assays for early events of influenza A virus (IAV) infection in tissue culture cells. IAVs are enveloped viruses belonging to the family Orthomyxoviridae with a negative-stranded, segmented RNA genome. To deliver their genome in the form of 8 viral ribonucleoproteins (vRNPs) into host cells, IAVs take advantage of the endocytic and cytosolic trafficking machinery of the host. After binding to sialic acid-containing receptors on the plasma membrane, IAV particles are internalized by clathrin-mediated endocytosis and macropinocytosis [1,2]. After sorting to late endosomes or mature macropinosomes, they are exposed to low pH (5.5?.0), whichinduces an irreversible conformational change in the viral hemagglutinin (HA, an envelope glycoprotein), activating its membrane fusion activity [3]. The viral envelope fuses with the limiting membrane of the endosome, and the capsid is released into the cytoplasm. The matrix protein M1 and the vRNPs dissociate from each other. The vRNPs are imported into the nucleus for transcription and replication of viral genes [4], whereas the M1 disperses into the cytosol (Figure 1a). High rates of mutation and the possibility of re-assortment facilitate generation of new IAV strains, decreasing the effect of vaccines and drugs. Therefore, instead of targeting the virus itself, it may be advantag.O increased in VHL-KO hepatocytes. The protein levels of GLUT1, one of the targets regulated by HIF-1a, were increased in VHL-inactivated hepatocytes; however, the GLUT 2 expression levels were comparable to those of the controls. Unexpectedly, inhibiting the IGF-IR signal with an IGF-IR antagonist sustained the blood glucose levels and remarkably suppressed the hepatic phenotypes. Therefore, the upregulated glucose transporters that were involved in VHL-KO mice were suspected to be GLUT1. In summary, VHL-KO mice exhibited severe hypoglycemia. Along with the severe hypoglycemia and hepatic glycogen accumulation in the liver, hepatic IGF-IR expression was upregulated and the IGF-IR-RACK1 interaction was enhanced. Administering an IGF-IR antagonist resulted in significant improvements in the hypoglycemic state and glycogen accumulation. Our results indicated that IGF-IR was a key regulator of the increased glucose uptake into VHL-deficient hepatocytes. To our knowledge, this is the first study to show that VHL-KO mice have increased glucose uptake into hepatocytes via IGF-IR activation, which contributes to their severe hypoglycemia.Author ContributionsConceived and designed the experiments: AK YK. Performed the experiments: AK YK. Analyzed the data: MF AK YK. Contributed reagents/materials/analysis tools: KI TS TM. Wrote the paper: AK YK.
In the field of infectious diseases, the use of high-content perturbation screens using siRNAs, shRNAs, and chemical agents is 18204824 rapidly expanding. Information regarding cellular factors that assist viruses and other intracellular pathogens during replication 1662274 in the host cell, and on pharmacological agents that affect infection is increasing. To understand disease mechanisms, and to develop novel antiviral strategies, it is important to precisely define the event in the viral replication cycle that is affected. Knowing the identity of a gene that promotes/inhibits infection, or a drug that blocks infection is not sufficient. Since the number of `hits’ provided by genome-wide and drug screens is generally large, such a method must be high-throughput. In this study, we describe a series of such assays for early events of influenza A virus (IAV) infection in tissue culture cells. IAVs are enveloped viruses belonging to the family Orthomyxoviridae with a negative-stranded, segmented RNA genome. To deliver their genome in the form of 8 viral ribonucleoproteins (vRNPs) into host cells, IAVs take advantage of the endocytic and cytosolic trafficking machinery of the host. After binding to sialic acid-containing receptors on the plasma membrane, IAV particles are internalized by clathrin-mediated endocytosis and macropinocytosis [1,2]. After sorting to late endosomes or mature macropinosomes, they are exposed to low pH (5.5?.0), whichinduces an irreversible conformational change in the viral hemagglutinin (HA, an envelope glycoprotein), activating its membrane fusion activity [3]. The viral envelope fuses with the limiting membrane of the endosome, and the capsid is released into the cytoplasm. The matrix protein M1 and the vRNPs dissociate from each other. The vRNPs are imported into the nucleus for transcription and replication of viral genes [4], whereas the M1 disperses into the cytosol (Figure 1a). High rates of mutation and the possibility of re-assortment facilitate generation of new IAV strains, decreasing the effect of vaccines and drugs. Therefore, instead of targeting the virus itself, it may be advantag.
Posted inUncategorized