Day: May 11, 2017

ture are incubated with the colorimetric substrate p-nitrophenyl-a-D-N-acetylglucosaminide. At pH 10, the product of hydrolysis, nitrophenyl, changes color and can be quantified spectrophotometrically at 420 nm. The intensity of absorbance,…

with the lentiviral luciferase construct and selected stable clones using puromycin. Clonal populations of cells were expanded in vitro and tested for their luciferase activities. Several bright clones were repeatedly…