Our previous results suggested that dsRNA originating from a plasmid suppresses the expression of co-transfected reporters in a sequence-independent manner
HEK-293 cells (,106106 mobile/dish) were transfected by pCAGEGFP or pCAGEGFP-MosIR plasmids making use of PEI and grown in ten cm dishes. Soon after 48 several hours, cells were washed with…