Each Cells were lysed by the addition of high salt lysis buffer directly to the plates as described in the previous section, and the lysates were processed as previously described
The major antibodies used were: goat polyclonal anti-topo I and goat polyclonal anti-ARF C-terminus (Santa Cruz Biotechnology, Santa Cruz, CA) mouse monoclonal anti-phosphoserine, mouse monoclonal anti-FLAG, and rabbit polyclonal antiFLAG…