Transfected THP1 cells were re-plated with selection media containing 10 mg/mL of puromycin (Calbiochem) to obtain cell colonies stably transfected with p62 shRNA plasmids
Twenty microliters of Protein G SepharoseTM Quickly Flow Beads (Sigma-Aldrich) have been added to lysates and incubated for an extra 1 h. Immunoabsorbents have been recovered by centrifugation at 10,000…