We determined Ogt in our Ring1B IP from ESCs nuclear extracts and this conversation was not impacted by mutation of I53A mutant model of Ring1B that abolishes the ubiquitnation catalytic action
The standard blanketing of H2A.Z at polycomb-controlled loci, even in Ring1B mutant cells, prompted us to re-take a look at the H2A.Z stages in cells lacking PRC2 operate. Mutation of…