To determine fluorescence at the maximal migration rate, equal numbers of cells were directly seeded to wells of the lower chamber while only medium was added to the upper chamber
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Expression cassettes for artificial micro RNAs (miRNAs) targeting NLRP3, ASC, and MyD88 and for negative control miRNAs were generated using the BLOCK-iT Pol II miR RNAi Expression Vector Kit
Ficoll-Paque Additionally was obtained from GE Health care Japan (Tokyo, Japan).Expression cassettes for artificial micro RNAs (miRNAs) targeting NLRP3, ASC, and MyD88 and for damaging handle miRNAs ended up generated…