Staining was visualized with three,39-diaminobenzidine (DAB Dako). The sections had been counterstained with hematoxylin (Dako). Sections had been ultimately washed with PBS, mounted, air-dried and deal with slipped with Aquamount (Dako)
Spearman correlations had been utilized to check out the connection among fold alter in autoantibody stages and end result steps.and held for five min just before returning to the preliminary…