For observation of the an infection method, selection of successfully replicating cell clones from HCV-RMT RNA-electroporated cells was needed. That clone, selected HuH7-K4-11 cells, experienced roughly 16108 copies/mg complete RNA of the HCV-RMT genome
Every single society of replicon RNA-electroporated cells was preserved and passaged with G418 variety for two months. Cell lysates (ten mg) were loaded onto an SDS-Web page gel. doi:10.1371/journal.pone.0082527.g00 We…