Proteins have been immunostained with the indicated primary antibodies for one h at place temperature. Detection of protein of curiosity was executed using peroxidaseconjugated secondary antibodies (Pierce, Rockford, IL) adopted
To much better visualize and optimally quantify the region of these neoplastic biliary ductules, a monoclonal antibody in opposition to cytokeratin, an epithelial-specific marker, was utilized utilizing immunohistochemistry staining and…