For the specific case of sort IV cytosolic phospholipase A2 (cPLA2), GM/TNF-dealt with cells had been stimulated with fMLP prior to the fractionation method in order to induce its phosphorylation and translocation to membranes
Analyses ended up executed on a JEOL JEM-1230 transmission electron microscope (JEOL, Montreal, QC, Canada). Magnifications had been among ten 0006 and one hundred twenty 0006. Immunoprecipitations with magnetic beads.Sodium…