Exudate samples were being then pooled and lyophilized. Lyophilized wound exudate samples were re-suspended in h2o and protein quantification was done utilizing NanoOrange protein kit (Invitrogen)
Briefly, a variety of amounts of every SVMP, dissolved in twenty mL of twenty five mM Tris, 150 mM NaCl, five mM CaCl2, pH 7.4, buffer have been incubated with…