The total reaction mix was loaded on to a silica-slim layer chromatography (TLC Analtech, Newark, DE) plate that was settled utilizing n-butanol-acetic acid-h2o (fifteen:five:8, by volume) as the solvent method
The facl6 (Rv1206) ORF corresponding to the protein shown in Fig. 1 was PCRamplified from the genomic DNA of Mtb H37Rv using Pfu Turbo HotStart DNA polymerase (Agilent Genomics, CA)…